A simple, specific, accurate, precise and reproducible Dual Wavelength method has been developed and validated for the simultaneous estimation of Aspirin (ASP) and Dipyridamole (DIP) in their combined dosage form. Measurements of absorbance were carried out at 285.80 and 299.00 nm for aspirin and 265.20 and 281.40 nm for dipyridamole by dual wavelength method. Beer’s law is obeyed in the concentration range of 1–17 μg/mL (r2=0.9998) for aspirin and 3–23 μg/mL (r2=0.9999) for dipyridamole. The method was successfully applied to pharmaceutical dosage form because no interference from the tablet excipients was found. The suitability of this method for quantitative determination of Aspirin and Dipyridamole was proved by validation and recovery studies. The method was validated as per International Conference on Harmonization (ICH) guideline in terms of accuracy, precision, linearity, limits of detection, limits of quantitation. This method has been successively applied to capsule formulation and no interference from the sample mixture’s excipients was found.
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